Biocaxis > Products > deoxynucleoside |
|
|||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||
Thymidine
Catalog NO.: D-N-004 | CAS NO.: 50-89-5 | Brand: BIOCAXIS
Category
Nucleosides & Nucleotides, 2’-deoxy
Synonyms:
1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil,
1-(2-Deoxy-β-D-ribofuranosyl)thymine, 2′-Deoxythymidine, Thymine
deoxyriboside, dT
Molecular Formula
C10H14N2O5
Molecular Weight
242.23
General description
General description
Thymidine is also referred to as pyrimidine
deoxynucleoside. Deoxythymidine is a nucleoside present in DNA. In a DNA double
stranded structure, thymidine pairs with deoxyadeninosine.
Thymidine, a specific precursor of deoxyribonucleic acid,
is used as a cell synchronizing agent. Thymidine is a DNA synthesis inhibitor
that can arrest cell at G1/S boundary, prior to DNA replication.
Application
Thymidine can be used in HT and HAT cocktails for hybridoma
fusion, selection and cloning. It has been used:
as a nucleotide source in minimal essential medium to
evaluate the outgrowth of the iSCNT-derived blastocysts
as a supplement in Dulbecco′s Modified Eagle Medium
(DMEM) selection medium used for the production of anti-neurogranin (NRGN)
antibodies
to prepare THMG (thymidine, hypoxanthine, glycine,
methotrexate) and THG stock solution for cleansing mouse lymphoma cells